![]() ![]() No cytotoxic effects of the GM coated surfaces were observed, giving rise to the conclusion that GMs are suitable materials as coatings in cell culture. Human fibroblasts cultured on the surfaces showed excellent viability shown by water soluble tetrazolium salt assay as well as live/dead staining with propidium iodide and fluorescein diacetate. Protein adsorption on the coated surfaces was reduced by the factor of 6.4 (uG), 5.4 (GM 1.5), and 4.6 (GM 4) compared to gold surfaces. Protein adsorption taking place on the coated surfaces was measured during contact of the surfaces with fetal calf serum by SPRS. The coated surfaces showed advancing contact angles of 46° (uG and GM 1.5) and 52° (GM 4) without alteration of the surface roughness determined by AFM. The authors found that upon adsorption of uG, GM 1.5, a nd GM 4 on gold, thin films with thicknesses of 2.95 nm, 2.50 nm, and 2.26 nm were formed. ![]() And the Jell-o your kids’ love comes from porcine gelatin which are collagen components of a pig’s connective. The great thing about it is that it can be transformed into different shapes and sizes. It is only obtained from approved animals and yes, it is safe for human consumption. ![]() Coating success was confirmed by spectroscopic ellipsometry, contact angle measurements, surface plasmon resonance spectroscopy (SPRS), and atomic force microscopy (AFM). To define, porcine gelatin comes from a pig’s collagen. Therefore, gold surfaces were coated with uG, GM 1.5, and GM 4 by adsorption of the polymers on the surfaces. In this study, the authors investigated if gelatin methacrylamide (GM) with a medium degree of methacrylamide modification (GM 1.5) and a high degree of methacrylamide modification (GM 4) are equally suitable for this purpose. Unmodified gelatin (uG) is widely used as a coating material in cell culture for improving surface properties. ![]()
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